Wong, J. E. M. M.
, Alsarraf, H. M. A. B., Kaspersen, J. D.
, Pedersen, J. S., Stougaard, J., Thirup, S. S. & Blaise, M. (2014).
Cooperative binding of LysM domains determines the carbohydrate affinity of a bacterial endopeptidase protein.
F E B S Journal,
281(4), 1196-1208.
https://doi.org/10.1111/febs.12698
Wong, J. E. M. M., Midtgaard, S. R.
, Gysel, K., Thygesen, M. B., Sørensen, K. K., Jensen, K. J.
, Stougaard, J., Thirup, S. & Blaise, M. (2015).
An intermolecular binding mechanism involving multiple LysM domains mediates carbohydrate recognition by an endopeptidase.
Acta crystallographica Section D: Structural biology ,
71(Pt 3), 592-605.
https://doi.org/10.1107/S139900471402793X
Wong, J. E. M. M.
, Nadzieja, M., Madsen, L. H., Bücherl, C. A., Dam, S.
, Sandal, N. N., Couto, D., Derbyshire, P., Uldum-Berentsen, M., Schroeder, S., Schwämmle, V., Nogueira, F. C. S.
, Asmussen, M. H., Thirup, S., Radutoiu, S., Blaise, M.
, Andersen, K. R., Menke, F. L. H., Zipfel, C.
& Stougaard, J. (2019).
A Lotus japonicus cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation.
Proceedings of the National Academy of Sciences,
116(28), 14339-14348.
https://doi.org/10.1073/pnas.1815425116
Vilstrup, J., Simonsen, A., Birkefeldt, T.
, Strandbygård, D., Lyngsø, J.
, Pedersen, J. S. & Thirup, S. (2020).
Crystal and solution structures of fragments of the human leucocyte common antigen-related protein.
Acta crystallographica Section D: Structural biology ,
76, 406-417.
https://doi.org/10.1107/S2059798320003885
Thirup, S. S., Petersen, C. M., Quistgaard, E. M. H.
, Madsen, P. S., Nissen, P., Grøftehauge, M. K., Cramer, J. F. & Andersen, J. L. (2010).
Design of specific ligands to Sortilin. (Patent No.
PA 2008 00592).
Thirup, S. S., Grøftehauge, M. K.
, Madsen, P. S., Petersen, C. M., Nissen, P., Quistgaard, E. M. H., Andersen, J. L. & Cramer, J. F. (2010).
Design and use of Sortilin specific molecular imaging ligands. (Patent No.
PA 2008 00593).
Schrøder, T. J., Christensen, S., Lindberg, S., Langgård, M., David, L., Maltas, P. J., Eskildsen, J., Jacobsen, J., Tagmose, L., Simonsen, K. B., Biilmann Rønn, L. C., de Jong, I. E. M., Malik, I. J., Karlsson, J.-J., Bundgaard, C., Egebjerg, J., Stavenhagen, J. B.
, Strandbygård, D., Thirup, S. S. ... Watson, S. P. (2014).
The identification of AF38469: An orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin: An orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin.
Bioorganic & Medicinal Chemistry Letters,
24(1), 177-180.
https://doi.org/10.1016/j.bmcl.2013.11.046
Radutoiu, S., Madsen, L. H., Madsen, E. B., Jurkiewicz, A., Fukai, E., Quistgaard, E. M. H., Albrektsen, A. S., James, E. K.
, Thirup, S. & Stougaard, J. (2007).
LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range. E M B O Journal,
26(17), 3923-35.
https://doi.org/10.1038/sj.emboj.7601826
Quistgaard, E. M.
, Madsen, P., Grøftehauge, M. K.
, Nissen, P., Petersen, C. M. & Thirup, S. S. (2009).
Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain.
Nature Structural and Molecular Biology,
16(1), 96-8.
https://doi.org/10.1038/nsmb.1543
Quistgaard, E. M., Grøftehauge, M. K.
, Madsen, P., Pallesen, L. T., Christensen, B. S., Sørensen, E. S., Nissen, P., Petersen, C. M. & Thirup, S. S. (2014).
Revisiting the structure of the Vps10 domain of human sortilin and its interaction with neurotensin.
Protein Science,
23(9), 1291-1300.
https://doi.org/10.1002/pro.2512
Polekhina, G.
, Thirup, S., Kjeldgaard, M.
, Nissen, P., Lippmann, C. & Nyborg, J. (1996).
Helix unwinding in the effector region of elongation factor EF-Tu-GDP.
Structure,
4(10), 1141-51.
Pallesen, L. T., Gustafsen, C., Cramer, J.
, Petersen, S. V., Thirup, S. S., Madsen, P. & Petersen, C. M. (2020).
PAK kinases targets sortilin and modulates its sorting.
Molecular and Cellular Biology,
40(3), Article e00411-19.
https://doi.org/10.1128/MCB.00411-19
Nyborg, J.
, Nissen, P., Kjeldgaard, M.
, Thirup, S., Polekhina, G., Clark, B. F. & Reshetnikova, L. (1997).
Macromolecular mimicry in protein biosynthesis.
Structure,
2(3), S7-11.
Nyborg, J.
, Nissen, P., Kjeldgaard, M.
, Thirup, S., Polekhina, G. & Clark, B. F. (1996).
Structure of the ternary complex of EF-Tu: macromolecular mimicry in translation.
Trends in Biochemical Sciences,
21(3), 81-2.
Nolsøe, S.
, Thirup, S., Etzerodt, M., Thøgersen, H. C. & Nyborg, J. (1997).
Crystallization and preliminary X-ray diffraction studies of psoriasin.
Acta Crystallographica Section D: Biological Crystallography,
53(1), 119-121.
https://doi.org/10.1107/S0907444996008955
Nolsø, S.
, Thirup, S. S., Etzerodt, M., Thøgersen, H. C. & Nyborg, J. (1997).
Crystallisation and preliminary X-ray diffraction studies of psoriasin.
Acta Crystallogr. D. Biol. Crystallogr.,
53(1), 119-121.
Nissen, P., Kjeldgaard, M.
, Thirup, S., Clark, B. F. & Nyborg, J. (1996).
The ternary complex of aminoacylated tRNA and EF-Tu-GTP. Recognition of a bond and a fold.
Biochimie,
78(11-12), 921-33.
Nissen, P., Kjeldgaard, M.
, Thirup, S., Polekhina, G., Reshetnikova, L., Clark, B. F. & Nyborg, J. (1995).
Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog.
Science,
270(5241), 1464-72.
Nissen, P., Reshetnikova, L., Siboska, G., Polekhina, G.
, Thirup, S., Kjeldgaard, M., Clark, B. F. & Nyborg, J. (1994).
Purification and crystallization of the ternary complex of elongation factor Tu:GTP and Phe-tRNA(Phe).
F E B S Letters,
356(2-3), 165-8.
Nielsen, M. J.
, Petersen, S. V., Jacobsen, C.
, Thirup, S. S., Enghild, J. J., Graversen, J. H.
& Moestrup, S. K. (2007).
A unique loop extension in the serine protease domain of haptoglobin is essential for CD163 recognition of the haptoglobin-hemoglobin complex.
Journal of Biological Chemistry,
282(2), 1072-1079.
Lohmann, G. V., Shimoda, Y., Nielsen, M. W., Jørgensen, F. G., Grossmann, C.
, Sandal, N., Sørensen, K.
, Thirup, S., Madsen, L. H., Tabata, S., Sato, S.
, Stougaard, J. & Radutoiu, S. (2010).
Evolution and regulation of the Lotus japonicus LysM receptor gene family.
Molecular plant-microbe interactions,
23(4), 510-21.
https://doi.org/10.1094/MPMI-23-4-0510
Kristensen, O., Reshetnikova, L.
, Nissen, P., Siboska, G.
, Thirup, S. & Nyborg, J. (1996).
Isolation, crystallization and X-ray analysis of the quaternary complex of Phe-tRNA(Phe), EF-Tu, a GTP analog and kirromycin.
F E B S Letters,
399(1-2), 59-62.
Kjolby, M., Pedersen, K. M., Breining, P., Wellner, N.
, Sørensen, K. M. J., Vittrup, D. M., Gjesing, A. M. P., Januliene, D., Manavalan, A., Rotman, M., Thotakura, G.
, Skeldal, S., Madsen, P.
, Thirup, S., Hermey, G.
, Vaegter, C. B., Möller, A., Asplund, O., Prasad, R. B.
... Nykjaer, A. (2020).
SorCS1 binds the insulin receptor to enhance insulin sensitivity. bioRxiv.
https://doi.org/10.1101/2020.11.10.376178
Kjærgaard, M., Rasmussen, L. S., Vinther, J. N., Andersen, K. R., Andersen, E. S., Lorentzen, E., Thirup, S. S., Otzen, D. & Brodersen, D. E. (2022).
A Semester-Long Learning Path Teaching Computational Skills via Molecular Graphics in PyMOL.
The Biophysicist,
3(2), 106–114.
https://doi.org/10.35459/tbp.2022.000219
Kawaharada, Y., Kelly, S., Nielsen, M. W., Hjuler, C. T.
, Gysel, K., Muszyński, A., Carlson, R. W., Thygesen, M. B.
, Sandal, N., Asmussen, M. H., Vinther, M., Andersen, S. U., Krusell, L.
, Thirup, S., Jensen, K. J., Ronson, C. W., Blaise, M.
, Radutoiu, S. & Stougaard, J. (2015).
Receptor-mediated exopolysaccharide perception controls bacterial infection.
Nature,
523, 308-312.
https://doi.org/10.1038/nature14611
Karring, H.
, Andersen, G. R., Thirup, S. S., Nyborg, J., Spremulli, L. L. & Clark, B. F. C. (2002).
Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.
Biochim. Biophys. Acta.,
1601(2), 172-177.
Karring, H., Björnsson, A.
, Thirup, S., Clark, B. F. C.
& Knudsen, C. R. (2003).
Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts.
F E B S Journal,
270, 4294-4305.
Johansen, J. S., Kavaliauskas, D., Pfeil, S., Blaise , M., Cooperman, B., Goldman, Y.
, Thirup, S. S. & Knudsen, C. R. (2018).
E. coli elongation factor Tu bound to a GTP analogue displays an open conformation equivalent to the GDP-bound form.
Nucleic Acids Research,
46(16), 8641-8650.
https://doi.org/10.1093/nar/gky697
Jenner, L., Husted, L.
, Thirup, S., Sottrup-Jensen, L. & Nyborg, J. (1998).
Crystal structure of the receptor-binding domain of alpha 2-macroglobulin.
Structure,
6(5), 595-604.
Januliene, D., Manavalan, A., Ovesen, P. L.
, Pedersen, K. M., Thirup, S., Nykjær, A. & Moeller, A. (2017).
Hidden Twins: SorCS Neuroreceptors Form Stable Dimers.
Journal of Molecular Biology,
429(19), 2907-2917.
https://doi.org/10.1016/j.jmb.2017.08.006
Januliene, D., Andersen, J. L., Nielsen, J. A., Quistgaard, E. M.
, Hansen, M., Strandbygaard, D., Moeller, A.
, Petersen, C. M., Madsen, P. & Thirup, S. S. (2017).
Acidic Environment Induces Dimerization and Ligand Binding Site Collapse in the Vps10p Domain of Sortilin.
Structure,
25(12), 1809-1819.e3. Article 12.
https://doi.org/10.1016/j.str.2017.09.015
Jankowski, V., Saritas, T.
, Kjolby, M., Hermann, J., Speer, T., Himmelsbach, A., Mahr, K., Heuschkel, M. A., Schunk, S. J.
, Thirup, S., Winther, S., Bottcher, M., Nyegard, M.
, Nykjaer, A., Kramann, R., Kaesler, N., Jankowski, J., Floege, J., Marx, N. & Goettsch, C. (2022).
Carbamylated sortilin associates with cardiovascular calcification in patients with chronic kidney disease.
Kidney International,
101(3), 574-584.
https://doi.org/10.1016/j.kint.2021.10.018
Gustafsen, C., Olsen, D., Vilstrup, J., Lund, S., Reinhardt, A., Wellner, N.
, Larsen, T., Andersen, C. B. F., Weyer, K., Li, J.-P., Seeberger, P. H.
, Thirup, S., Madsen, P. & Glerup, S. (2017).
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor.
Nature Communications,
8(1), 503. Article 503.
https://doi.org/10.1038/s41467-017-00568-7
Dolmer, K., Jenner, L. B., Jacobsen, L.
, Andersen, G. R., Koch, T. J.
, Thirup, S., Sottrup-Jensen, L. & Nyborg, J. (1995).
Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine alpha 2-macroglobulin.
F E B S Letters,
372(1), 93-5.
Discipio, R. G., Jenner, L.
, Thirup, S., Sottrup-Jensen, L., Nyborg, J. & Stura, E. (1998).
Crystallization of human complement component C5.
Acta Crystallographica. Section D: Biological Crystallography,
54(Pt 4), 643-6.
Cramer, J. F.
, Gustafsen, C., Behrens, M. A., Oliveira, C. L. P.
, Pedersen, J. S., Madsen, P., Petersen, C. M. & Thirup, S. S. (2010).
GGA autoinhibition revisited.
Traffic,
11(2), 259-73.
https://doi.org/10.1111/j.1600-0854.2009.01017.x
Clark, B. F., Kjeldgaard, M., la Cour, T. F.
, Thirup, S. & Nyborg, J. (1990).
Structural determination of the functional sites of E. coli elongation factor Tu.
BBA General Subjects,
1050(1-3), 203-8.
Broghammer, A., Krusell, L., Blaise, M.
, Thirup, S. S. & Stougaard, J. (2010).
Characterization of LysM-receptors and their ligands involved in development and regulation of legume-rhizobium symbiosis. Poster session presented at European Nitrogen Fixation Conference, Geneva, Switzerland.
Broghammer, A., Krusell, L., Blaise, M., Sauer, J., Sullivan, J. T., Maolanon, N. N.
, Vinther, M., Lorentzen, A. M., Madsen, E. B., Jensen, K. J., Roepstorff, P.
, Thirup, S. S., Ronson, C. W., Thygesen, M. B.
& Stougaard, J. (2012).
Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding.
Proceedings of the National Academy of Sciences,
109(34), 13859-13864.
https://doi.org/10.1073/pnas.1205171109
Blaise, M., Bailly, M., Frechin, M., Behrens, M. A., Fischer, F., Oliveira, C. L. P., Becker, H. D.
, Pedersen, J. S., Thirup, S. & Kern, D. (2010).
Crystal structure of a transfer-ribonucleoprotein particle that promotes asparagine formation.
The EMBO Journal,
29(18), 3118-29.
https://doi.org/10.1038/emboj.2010.192