Kaas, M., Dinesen, S. B., Ahlgreen, O., Madsen, P., Mølgaard, S., Dalby, A.
, Gustafsen, C., Olsen, D., Duan, J., Vilstrup, J., Lende, J., Nordestgaard, S., Zayats, T., Knappskog, P. M., Johansson, S., Neckelmann, G., Franke, B.
, Thirup, S., Børglum, A. ... Skeldal, S. (2026).
A low frequency damaging SORCS2 variant identified in a family with ADHD compromises receptor stability and quenches activity.
Molecular Psychiatry,
31(3), 1311-1324.
https://doi.org/10.1038/s41380-025-03242-3
Kaas, M., Chofflet, N.
, Bicer, D., Skeldal, S.
, Duan, J., Feller, B.
, Vilstrup, J., Groth, R., Sivagurunathan, S., Dashti, H.
, Pedersen, J. S., Werge, T.
, Børglum, A. D., Cimini, B. A., Jones, T. R., Claussnitzer, M.
, Madsen, P., Takahashi, H.
, Demontis, D. ... Glerup, S. (2025).
Rare missense variants of the leukocyte common antigen related receptor (LAR) display reduced activity in transcellular adhesion and synapse formation. Research Square.
https://doi.org/10.1101/2025.02.16.638491,
https://doi.org/10.21203/rs.3.rs-5928514/v1
Mehmood, S.
, Thirup, S. S., Ahmed, S., Bashir, N., Saeed, A., Rafiq, M., Saeed, Q., Najam-ul-Haq, M., Khaliq, B., Ibrahim, M., Alonazi, W. B. & Akrem, A. (2024).
Crystal structure of Kunitz-type trypsin inhibitor: Entomotoxic effect of native and encapsulated protein targeting gut trypsin of Tribolium castaneum Herbst.
Computational and Structural Biotechnology Journal,
23, 3132-3142.
https://doi.org/10.1016/j.csbj.2024.07.023Kjærgaard, M., Rasmussen, L. S., Vinther, J. N., Andersen, K. R., Andersen, E. S., Lorentzen, E., Thirup, S. S., Otzen, D. & Brodersen, D. E. (2022).
A Semester-Long Learning Path Teaching Computational Skills via Molecular Graphics in PyMOL.
The Biophysicist,
3(2), 106–114.
https://doi.org/10.35459/tbp.2022.000219
Jankowski, V., Saritas, T.
, Kjolby, M., Hermann, J., Speer, T., Himmelsbach, A., Mahr, K., Heuschkel, M. A., Schunk, S. J.
, Thirup, S., Winther, S., Bottcher, M., Nyegard, M.
, Nykjaer, A., Kramann, R., Kaesler, N., Jankowski, J., Floege, J., Marx, N. & Goettsch, C. (2022).
Carbamylated sortilin associates with cardiovascular calcification in patients with chronic kidney disease.
Kidney International,
101(3), 574-584.
https://doi.org/10.1016/j.kint.2021.10.018Vilstrup, J., Simonsen, A., Birkefeldt, T., Strandbygård, D., Lyngsø, J., Pedersen, J. S. & Thirup, S. (2020).
Crystal and solution structures of fragments of the human leucocyte common antigen-related protein.
Acta Crystallographica Section D: Structural Biology,
76, 406-417.
https://doi.org/10.1107/S2059798320003885
Pallesen, L. T., Gustafsen, C., Cramer, J., Petersen, S. V., Thirup, S. S., Madsen, P. & Petersen, C. M. (2020).
PAK kinases targets sortilin and modulates its sorting.
Molecular and Cellular Biology,
40(3), Article e00411-19.
https://doi.org/10.1128/MCB.00411-19
Kjolby, M., Pedersen, K. M., Breining, P., Wellner, N., Sørensen, K. M. J., Vittrup, D. M., Gjesing, A. M. P.
, Januliene, D., Manavalan, A., Rotman, M., Thotakura, G., Skeldal, S., Madsen, P.
, Thirup, S., Hermey, G.
, Vaegter, C. B., Möller, A., Asplund, O., Prasad, R. B.
... Nykjaer, A. (2020).
SorCS1 binds the insulin receptor to enhance insulin sensitivity. bioRxiv.
https://doi.org/10.1101/2020.11.10.376178
Wong, J. E. M. M., Nadzieja, M., Madsen, L. H., Bücherl, C. A.
, Dam, S., Sandal, N. N., Couto, D., Derbyshire, P.
, Uldum-Berentsen, M., Schroeder, S., Schwämmle, V., Nogueira, F. C. S.
, Asmussen, M. H., Thirup, S., Radutoiu, S., Blaise, M., Andersen, K. R., Menke, F. L. H., Zipfel, C.
& Stougaard, J. (2019).
A Lotus japonicus cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation.
Proceedings of the National Academy of Sciences (PNAS),
116(28), 14339-14348.
https://doi.org/10.1073/pnas.1815425116
Johansen, J. S., Kavaliauskas, D., Pfeil, S.
, Blaise , M., Cooperman, B., Goldman, Y.
, Thirup, S. S. & Knudsen, C. R. (2018).
E. coli elongation factor Tu bound to a GTP analogue displays an open conformation equivalent to the GDP-bound form.
Nucleic Acids Research,
46(16), 8641-8650.
https://doi.org/10.1093/nar/gky697
Januliene, D., Andersen, J. L., Nielsen, J. A., Quistgaard, E. M., Hansen, M., Strandbygaard, D., Moeller, A.
, Petersen, C. M., Madsen, P. & Thirup, S. S. (2017).
Acidic Environment Induces Dimerization and Ligand Binding Site Collapse in the Vps10p Domain of Sortilin.
Structure,
25(12), 1809-1819.e3. Article 12.
https://doi.org/10.1016/j.str.2017.09.015
Gustafsen, C., Olsen, D., Vilstrup, J., Lund, S., Reinhardt, A.
, Wellner, N., Larsen, T., Andersen, C. B. F., Weyer, K., Li, J.-P., Seeberger, P. H.
, Thirup, S., Madsen, P. & Glerup, S. (2017).
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor.
Nature Communications,
8(1), 503. Article 503.
https://doi.org/10.1038/s41467-017-00568-7
Januliene, D., Manavalan, A., Ovesen, P. L., Pedersen, K. M., Thirup, S., Nykjær, A. & Moeller, A. (2017).
Hidden Twins: SorCS Neuroreceptors Form Stable Dimers.
Journal of Molecular Biology,
429(19), 2907-2917.
https://doi.org/10.1016/j.jmb.2017.08.006
Andersen, J. L., Lindberg, S., Langgård, M., Maltas, P. J., Rønn, L. C. B., Bundgaard, C.
, Strandbygaard, D., Thirup, S. & Watson, S. P. (2017).
The identification of novel acid isostere based inhibitors of the VPS10P family sorting receptor Sortilin.
Bioorganic & Medicinal Chemistry Letters,
27(11), 2629-2633.
https://doi.org/10.1016/j.bmcl.2017.02.028
Wong, J. E. M. M., Midtgaard, S. R.
, Gysel, K., Thygesen, M. B., Sørensen, K. K., Jensen, K. J., Stougaard, J., Thirup, S. & Blaise, M. (2015).
An intermolecular binding mechanism involving multiple LysM domains mediates carbohydrate recognition by an endopeptidase.
Acta Crystallographica Section D: Structural Biology,
71(Pt 3), 592-605.
https://doi.org/10.1107/S139900471402793X
Kawaharada, Y., Kelly, S., Nielsen, M. W., Hjuler, C. T.
, Gysel, K., Muszyński, A., Carlson, R. W.
, Thygesen, M. B., Sandal, N., Asmussen, M. H., Vinther, M., Andersen, S. U., Krusell, L., Thirup, S., Jensen, K. J., Ronson, C. W.
, Blaise, M., Radutoiu, S. & Stougaard, J. (2015).
Receptor-mediated exopolysaccharide perception controls bacterial infection.
Nature,
523, 308-312.
https://doi.org/10.1038/nature14611
Wong, J. E. M. M., Alsarraf, H. M. A. B., Kaspersen, J. D., Pedersen, J. S., Stougaard, J., Thirup, S. S. & Blaise, M. (2014).
Cooperative binding of LysM domains determines the carbohydrate affinity of a bacterial endopeptidase protein.
FEBS journal,
281(4), 1196-1208.
https://doi.org/10.1111/febs.12698
Andersen, J. L., Schrøder, T. J., Christensen, S.
, Strandbygård, D. J., Pallesen, L. T., Garcia Alai, M. M., Lindberg, S., Langgård, M., Eskildsen, J. C., David, L., Tagmose, L., Simonsen, K. B., Maltas, P. J., Rønn, L. C. B., de Jong, I. E. M., Malik, I. J., Egebjerg, J., Karlsson, J. J., Uppalanchi, S.
... Thirup, S. (2014).
Identification of the first small-molecule ligand of the neuronal receptor sortilin and structure determination of the receptor-ligand complex.
Acta Crystallographica Section D: Biological Crystallography,
70(Part 2), 451-460.
https://doi.org/10.1107/S1399004713030149
Quistgaard, E. M., Grøftehauge, M. K., Madsen, P., Pallesen, L. T., Christensen, B. S., Sørensen, E. S., Nissen, P., Petersen, C. M. & Thirup, S. S. (2014).
Revisiting the structure of the Vps10 domain of human sortilin and its interaction with neurotensin.
Protein Science,
23(9), 1291-1300.
https://doi.org/10.1002/pro.2512
Schrøder, T. J., Christensen, S., Lindberg, S., Langgård, M., David, L., Maltas, P. J., Eskildsen, J., Jacobsen, J., Tagmose, L., Simonsen, K. B., Biilmann Rønn, L. C., de Jong, I. E. M., Malik, I. J., Karlsson, J.-J., Bundgaard, C., Egebjerg, J., Stavenhagen, J. B.
, Strandbygård, D., Thirup, S. S. ... Watson, S. P. (2014).
The identification of AF38469: An orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin: An orally bioavailable inhibitor of the VPS10P family sorting receptor Sortilin.
Bioorganic & Medicinal Chemistry Letters,
24(1), 177-180.
https://doi.org/10.1016/j.bmcl.2013.11.046Blaise, M., Alsarraf, H. M. A. B., Wong, J., Roi Midtgaard, S.
, Laroche, F. J. F., Schack, L., Spaink, H.
, Stougaard, J. & Thirup, S. S. (2012).
Crystal structure of the TLDc domain of oxidation resistance protein 2 from zebrafish.
Proteins: Structure, Function, and Bioinformatics,
80(6), 1694-1698.
https://doi.org/10.1002/prot.24050
Broghammer, A., Krusell, L., Blaise, M., Sauer, J., Sullivan, J. T., Maolanon, N. N.
, Vinther, M., Lorentzen, A. M.
, Madsen, E. B., Jensen, K. J., Roepstorff, P.
, Thirup, S. S., Ronson, C. W.
, Thygesen, M. B. & Stougaard, J. (2012).
Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding.
Proceedings of the National Academy of Sciences (PNAS),
109(34), 13859-13864.
https://doi.org/10.1073/pnas.1205171109
Alsarraf, H. M. A. B., Laroche, F. J. F., Spaink, H.
, Thirup, S. S. & Blaise, M. (2011).
Purification, crystallization and preliminary crystallographic studies of the TLDc domain of oxidation resistance protein 2 from zebrafish.
Acta Crystallographica Section F:Structural Biology Communications,
67(10).
Broghammer, A., Krusell, L., Blaise, M., Thirup, S. S. & Stougaard, J. (2010).
Characterization of LysM-receptors and their ligands involved in development and regulation of legume-rhizobium symbiosis. Poster session presented at European Nitrogen Fixation Conference, Geneva, Switzerland.
Blaise, M., Bailly, M., Frechin, M.
, Behrens, M. A., Fischer, F.
, Oliveira, C. L. P., Becker, H. D.
, Pedersen, J. S., Thirup, S. & Kern, D. (2010).
Crystal structure of a transfer-ribonucleoprotein particle that promotes asparagine formation.
EMBO Journal,
29(18), 3118-29.
https://doi.org/10.1038/emboj.2010.192
Thirup, S. S., Grøftehauge, M. K., Madsen, P. S., Petersen, C. M., Nissen, P., Quistgaard, E. M. H., Andersen, J. L. & Cramer, J. F. (2010).
Design and use of Sortilin specific molecular imaging ligands. (Patent No.
PA 2008 00593).
Thirup, S. S., Petersen, C. M., Quistgaard, E. M. H., Madsen, P. S., Nissen, P., Grøftehauge, M. K., Cramer, J. F. & Andersen, J. L. (2010).
Design of specific ligands to Sortilin. (Patent No.
PA 2008 00592).
Lohmann, G. V., Shimoda, Y.
, Nielsen, M. W., Jørgensen, F. G., Grossmann, C., Sandal, N., Sørensen, K., Thirup, S., Madsen, L. H., Tabata, S., Sato, S.
, Stougaard, J. & Radutoiu, S. (2010).
Evolution and regulation of the Lotus japonicus LysM receptor gene family.
Molecular plant-microbe interactions,
23(4), 510-21.
https://doi.org/10.1094/MPMI-23-4-0510
Cramer, J. F., Gustafsen, C., Behrens, M. A., Oliveira, C. L. P., Pedersen, J. S., Madsen, P., Petersen, C. M. & Thirup, S. S. (2010).
GGA autoinhibition revisited.
Traffic,
11(2), 259-73.
https://doi.org/10.1111/j.1600-0854.2009.01017.x
Bek, A. S., Sauer, J., Thygesen, M. B., Duus, J. Ø., Petersen, B. O.
, Thirup, S., James, E., Jensen, K. J.
, Stougaard, J. & Radutoiu, S. (2010).
Improved Characterization of Nod Factors and Genetically Based Variation in LysM Receptor Domains Identify Amino Acids Expendable for Nod Factor Recognition in Lotus spp.
Molecular plant-microbe interactions,
23(1), 58-66.
https://doi.org/10.1094/MPMI-23-1-0058
Bailly, M.
, Blaise, M., Lorber, B.
, Thirup, S. S. & Kern, D. (2009).
Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation.
Acta Crystallographica Section F:Structural Biology Communications,
65(Pt 6), 577-81.
https://doi.org/10.1107/S1744309109015000Quistgaard, E. M., Madsen, P., Grøftehauge, M. K., Nissen, P., Petersen, C. M. & Thirup, S. S. (2009).
Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain.
Nature Structural and Molecular Biology,
16(1), 96-8.
https://doi.org/10.1038/nsmb.1543
Nielsen, M. J., Petersen, S. V., Jacobsen, C., Thirup, S. S., Enghild, J. J., Graversen, J. H.
& Moestrup, S. K. (2007).
A unique loop extension in the serine protease domain of haptoglobin is essential for CD163 recognition of the haptoglobin-hemoglobin complex.
Journal of Biological Chemistry,
282(2), 1072-1079.
Radutoiu, S., Madsen, L. H., Madsen, E. B., Jurkiewicz, A., Fukai, E., Quistgaard, E. M. H., Albrektsen, A. S., James, E. K.
, Thirup, S. & Stougaard, J. (2007).
LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range. EMBO Journal,
26(17), 3923-35.
https://doi.org/10.1038/sj.emboj.7601826
Karring, H., Björnsson, A.
, Thirup, S., Clark, B. F. C. & Knudsen, C. R. (2003).
Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts.
FEBS journal,
270, 4294-4305.
Karring, H., Andersen, G. R., Thirup, S. S., Nyborg, J., Spremulli, L. L.
& Clark, B. F. C. (2002).
Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.
Biochim. Biophys. Acta.,
1601(2), 172-177.
Andersen, G. R., Stepanov, V. G.
, Kjeldgaard, M., Thirup, S. S., Nyborg, J., Garrett, R. A. (Ed.), Douthwaite, S. R. (Ed.), Liljas, A. (Ed.), Matheson, A. T. (Ed.), Moore, P. B. (Ed.) & Noller, H. F. (Ed.) (2000).
Ternary complex of EF-Tu and its action on the ribosome. In
The Ribosome: Structure, Function, Antibiotics, and Cellular Interactions (pp. 337-345). ASM Press.
Discipio, R. G.
, Jenner, L., Thirup, S., Sottrup-Jensen, L., Nyborg, J. & Stura, E. (1998).
Crystallization of human complement component C5.
Acta Crystallographica Section D: Biological Crystallography,
54(Pt 4), 643-6.
Jenner, L., Husted, L., Thirup, S., Sottrup-Jensen, L. & Nyborg, J. (1998).
Crystal structure of the receptor-binding domain of alpha 2-macroglobulin.
Structure,
6(5), 595-604.
Nolsø, S.
, Thirup, S. S., Etzerodt, M., Thøgersen, H. C. & Nyborg, J. (1997).
Crystallisation and preliminary X-ray diffraction studies of psoriasin.
Acta Crystallographica Section D: Structural Biology,
53(1), 119-121.
Nolsøe, S.
, Thirup, S., Etzerodt, M., Thøgersen, H. C. & Nyborg, J. (1997).
Crystallization and preliminary X-ray diffraction studies of psoriasin.
Acta Crystallographica Section D: Biological Crystallography,
53(1), 119-121.
https://doi.org/10.1107/S0907444996008955Nyborg, J., Nissen, P., Kjeldgaard, M., Thirup, S., Polekhina, G.
, Clark, B. F. & Reshetnikova, L. (1997).
Macromolecular mimicry in protein biosynthesis.
Structure,
2(3), S7-11.
Polekhina, G.
, Thirup, S., Kjeldgaard, M., Nissen, P., Lippmann, C.
& Nyborg, J. (1996).
Helix unwinding in the effector region of elongation factor EF-Tu-GDP.
Structure,
4(10), 1141-51.
Kristensen, O., Reshetnikova, L.
, Nissen, P., Siboska, G., Thirup, S. & Nyborg, J. (1996).
Isolation, crystallization and X-ray analysis of the quaternary complex of Phe-tRNA(Phe), EF-Tu, a GTP analog and kirromycin.
FEBS Letters,
399(1-2), 59-62.
